BLNK Monoklonaler Antikörper

BLNK Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1A1

Kat-Nr. : 66953-1-Ig

Synonyme

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

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  WB analysis of human spleen using 66953-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 66953-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 66953-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66953-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66953-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 66953-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 66953-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of NIH/3T3 using 66953-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, Daudi-Zellen, Hausschwein-Milzgewebe, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, Maus-Thymusgewebe, Rattenmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66953-1-Ig bindet in WB, IHC, IF/ICC, ELISA BLNK und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BLNK fusion protein Ag28673
• Vollständiger Name: B-cell linker
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC018906
• Gene symbol: BLNK
• Gene ID (NCBI): 29760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.