BMP5 Polyklonaler Antikörper

BMP5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 13253-1-AP

Synonyme

BMP 5, BMP5, BMP-5, bone morphogenetic protein 5

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  WB analysis of mouse lung using 13253-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13253-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 13253-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13253-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

13253-1-AP bindet in WB, IHC, ELISA BMP5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMP5 fusion protein Ag3952
• Vollständiger Name: bone morphogenetic protein 5
• Berechnetes Molekulargewicht: 454 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC027958
• Gene symbol: BMP5
• Gene ID (NCBI): 653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; G9a Knockdown Suppresses Cancer Aggressiveness by Facilitating Smad Protein Phosphorylation through Increasing BMP5 Expression in Luminal A Type Breast Cancer.; Authors - Yunho Jin
human	WB	FASEB J; Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis; Authors - Xiang Gao
human	WB	Placenta; BMP5 promotes trophoblast functions upon N-glycosylation via the BMP5-SMAD1/5 signaling pathway in preeclampsia; Authors - Hao Wang
	WB	Phytomedicine; Lutein protects senescent ciliary muscle against oxidative stress through the Keap1/Nrf2/ARE pathway; Authors - Ning Gao
human	IHC	Mol Cancer; Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.; Authors - Erfei Chen