BMP7 Polyklonaler Antikörper

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• KD/KO Validated

BMP7 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 12221-1-AP

Synonyme

BMP 7, BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1

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Galerie der Validierungsdaten

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  WB analysis using 12221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12221-1-AP (BMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12221-1-AP

  WB result of BMP7 antibody (12221-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMP7 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 12221-1-AP

  IP Result of anti-BMP7 (IP:12221-1-AP, 4ug; Detection:12221-1-AP 1:800) with HEK-293 cells lysate 2800ug.

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  IHC staining of mouse brain using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12221-1-AP (BMP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12221-1-AP (BMP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bladder using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12221-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BMP7 antibody (12221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Blasengewebe, humanes Nierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Produktinformation

12221-1-AP bindet in WB, IP, IHC, IF, ELISA BMP7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMP7 fusion protein Ag2902
• Vollständiger Name: bone morphogenetic protein 7
• Berechnetes Molekulargewicht: 431 aa, 49 kDa
• Beobachtetes Molekulargewicht: 43-49 kDa
• GenBank-Zugangsnummer: BC008584
• Gene symbol: BMP7
• Gene ID (NCBI): 655
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

Publikationen

Species	Application	Title
	WB	Circ Res; Yin Yang 1 Suppresses Dilated Cardiomyopathy and Cardiac Fibrosis Through Regulation of Bmp7 and Ctgf.; Authors - Chia Yee Tan
	WB,IF	Cell Mol Life Sci; Omentin-1 drives cardiomyocyte cell cycle arrest and metabolic maturation by interacting with BMP7; Authors - Huijun Yang
rat	WB	Nanoscale; Concentrated small extracellular vesicles from menstrual blood-derived stromal cells improve intrauterine adhesion, a pre-clinical study in a rat model.; Authors - Siwen Zhang
	WB	Front Mol Neurosci; Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.; Authors - Tianda Chen
rat	WB,IHC,IF	Front Pharmacol; BMP-7 Upregulates Id2 Through the MAPK Signaling Pathway to Improve Diabetic Tubulointerstitial Fibrosis and the Intervention of Oxymatrine.; Authors - Yawen Xiao
	WB	BMC Complement Med Ther; Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway; Authors - Ying Chen