BMP9 Rekombinanter Antikörper

BMP9 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250512B9

Kat-Nr. : 86411-1-PBS

Synonyme

BMP-9, Bone morphogenetic protein 9, Gdf2, GDF-2, Growth/differentiation factor 2

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  WB analysis using 86411-1-RR (same clone as 86411-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86411-1-RR (Gdf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86411-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02608-1

  The mean BMP9 concentration was determined to be 106.40 ng/mL in mouse liver tissue extract based on a 8.40 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02608-1

  Sandwich ELISA standard curve of MP02608-1, Mouse BMP9 Recombinant Matched Antibody Pair - PBS only. 86411-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2482. 86411-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86411-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA BMP9 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BMP9 fusion protein Eg2482
• Vollständiger Name: growth differentiation factor 2
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_019506.4
• Gene symbol: BMP9
• Gene ID (NCBI): 12165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bone morphogenetic protein-9 (BMP9, also termed growth-differentiation factor-2, GDF-2) is a secreted signaling molecule that belongs to the transforming growth factor-β (TGF-β) super-family. First identified in fetal mouse liver cDNA libraries, BMP9 is predominantly produced by hepatic stellate cells and circulates at relatively high concentrations in adult plasma. BMP9 is synthesized as a precursor protein (pre-proBMP9) composed of 429 amino acids (aa) that includes a 22 aa signal peptide, a 297 aa prodomain (33 kDa) and a 110 aa mature protein (12.5 kDa). It is then cleaved by serine endoproteases, leading to a short dimeric mature form of 25 kDa, and the prodomain which can remain noncovalently associated with the mature short form, giving a size of about 100 kDa (PMID:21710321).