BMPR1A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BMPR1A Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 12702-1-AP

Synonyme

Activin receptor like kinase 3, Activin receptor-like kinase 3, ACVRLK3, ALK 3, ALK3

---

• 

  WB analysis using 12702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12702-1-AP

  WB result of BMPR1A antibody (12702-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMPR1A transfected MCF-7 cells.

• 

  WB analysis of human skeletal muscle using 12702-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12702-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12702-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12702-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12702-1-AP

  IP result of anti-BMPR1A (IP:12702-1-AP, 4ug; Detection:12702-1-AP 1:500) with HEK-293 cells lysate 2000ug.

• 

  IF Staining of HeLa using 12702-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BMPR1A antibody (12702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of U2OS using 12702-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using BMPR1A antibody (12702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, Mauslebergewebe, U2OS-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

12702-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BMPR1A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMPR1A fusion protein Ag3410
• Vollständiger Name: bone morphogenetic protein receptor, type IA
• Berechnetes Molekulargewicht: 532 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-68 kDa
• GenBank-Zugangsnummer: BC028383
• Gene symbol: BMPR1A
• Gene ID (NCBI): 657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo
human	IP	Sci Signal; Reverse signaling by semaphorin 4C elicits SMAD1/5- and ID1/3-dependent invasive reprogramming in cancer cells.; Authors - Sreeharsha Gurrapu
mouse	WB,IF	Front Cell Dev Biol; Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland.; Authors - Chunlei Shao
mouse	WB	PLoS One; Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro.; Authors - Shasha Zhu
human	WB	Biochim Biophys Acta Gen Subj; Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels; Authors - Tianmiao Huang
rat	WB	Sci Rep; Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.; Authors - Yan Zeng