BMPR2 Polyklonaler Antikörper

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• KD/KO Validated

BMPR2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19087-1-PBS

Synonyme

BMPR-2, BMPR II, BMPR 2, BMP type-2 receptor, BMP type II receptor

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  WB analysis of human heart using 19087-1-AP (same clone as 19087-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 19087-1-AP (BMPR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19087-1-AP (BMPR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

  IP result of anti-BMPR2 (IP:19087-1-AP, 4ug; Detection:19087-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 19087-1-AP (same clone as 19087-1-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug BMPR2 Polyclonal antibody (19087-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19087-1-PBS bindet in WB, IHC, IP, Indirect ELISA BMPR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMPR2 fusion protein Ag5868
• Vollständiger Name: bone morphogenetic protein receptor, type II (serine/threonine kinase)
• Berechnetes Molekulargewicht: 115 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC052985
• Gene symbol: BMPR2
• Gene ID (NCBI): 659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. The dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form, and 150 kD dimer.