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BNIP1 Polyklonaler Antikörper

BNIP1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15964-1-AP

Synonyme

BNIP1, NIP1, SEC20, SEC20L, TRG 8

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using BNIP1 Polyclonal antibody (15964-1-AP)

    WB analysis using 15964-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15964-1-AP (BNIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BNIP1 Polyclonal antibody (15964-1-AP)

    IP experiment of mouse brain using 15964-1-AP

    IP Result of anti-BNIP1 (IP:15964-1-AP, 3ug; Detection:15964-1-AP 1:500) with mouse brain tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using BNIP1 Polyclonal antibody (15964-1-AP)

    IHC staining of human heart using 15964-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using BNIP1 Polyclonal antibody (15964-1-AP)

    IHC staining of human heart using 15964-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, Maus-Skelettmuskelgewebe, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15964-1-AP bindet in WB, IP, IHC, IF, ELISA BNIP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BNIP1 fusion protein Ag8737
Vollständiger Name BCL2/adenovirus E1B 19kDa interacting protein 1
Berechnetes Molekulargewicht 228 aa, 26 kDa
Beobachtetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerBC010959
Gene symbol BNIP1
Gene ID (NCBI) 662
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.

Protokolle

Produktspezifische Protokolle
WB protocol for BNIP1 antibody 15964-1-APProtokoll herunterladen
IHC protocol for BNIP1 antibody 15964-1-APProtokoll herunterladen
IP protocol for BNIP1 antibody 15964-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IP

Cell Signal

A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.

Authors - Wang Peng P
View Article
humanWB,IF

Hum Mutat

A homozygous hypomorphic BNIP1 variant causes an increase in autophagosomes and reduced autophagic flux and results in a spondylo-epiphyseal dysplasia.

Authors - Tess Holling
View Article
humanWB,IF

PLoS One

RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1.

Authors - Tang Fei F
View Article
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article