BNIP1 Polyklonaler Antikörper

BNIP1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15964-1-PBS

Synonyme

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  WB analysis using 15964-1-AP (same clone as 15964-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15964-1-AP (BNIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 15964-1-AP (same clone as 15964-1-PBS)

  IP result of anti-BNIP1 (IP:15964-1-AP, 3ug; Detection:15964-1-AP 1:500) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15964-1-PBS bindet in WB, IHC, IP, Indirect ELISA BNIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BNIP1 fusion protein Ag8737
• Vollständiger Name: BCL2/adenovirus E1B 19kDa interacting protein 1
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC010959
• Gene symbol: BNIP1
• Gene ID (NCBI): 662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.