BNIP3 Monoklonaler Antikörper
BNIP3 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2H7D8
Kat-Nr. : 68091-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HepG2-Zellen, LNCaP-Zellen, NIH/3T3-Zellen |
Erfolgreiche Detektion in IHC | Maushirngewebe, Mausnierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | mit Cobaltchlorid behandelte HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 30 publications below |
IHC | See 7 publications below |
IF | See 7 publications below |
CoIP | See 1 publications below |
Produktinformation
68091-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA BNIP3 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | BNIP3 fusion protein Ag27935 |
Vollständiger Name | BCL2/adenovirus E1B 19kDa interacting protein 3 |
Berechnetes Molekulargewicht | 194 aa, 22 kDa |
Beobachtetes Molekulargewicht | 25-30,50-60 kDa |
GenBank-Zugangsnummer | BC009342 |
Gene symbol | BNIP3 |
Gene ID (NCBI) | 664 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for BNIP3 antibody 68091-1-Ig | Protokoll herunterladen |
IHC protocol for BNIP3 antibody 68091-1-Ig | Protokoll herunterladenl |
IF protocol for BNIP3 antibody 68091-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells | ||
Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy | ||
Cell Biosci Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy | ||
Cell Rep Proline restores mitochondrial function and reverses aging hallmarks in senescent cells | ||
Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma | ||
Cell Commun Signal Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1 |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Paula (Verified Customer) (07-26-2024) | Worked well for Western Blotting
|
FH Aidan (Verified Customer) (04-10-2024) | worked v well, would highly recommend. Incubated overnight 4 degrees 1:5000.
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