BNIP3 Monoklonaler Antikörper

BNIP3 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H7D8

Kat-Nr. : 68091-1-PBS

Synonyme

2H7D8, NIP3

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  WB analysis of NIH/3T3 using 68091-1-Ig (same clone as 68091-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68091-1-Ig (same clone as 68091-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68091-1-PBS bindet in WB, IHC, IF/ICC, ELISA BNIP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BNIP3 fusion protein Ag27935
• Vollständiger Name: BCL2/adenovirus E1B 19kDa interacting protein 3
• Berechnetes Molekulargewicht: 194 aa, 22 kDa
• Beobachtetes Molekulargewicht: 25-30,50-60 kDa
• GenBank-Zugangsnummer: BC009342
• Gene symbol: BNIP3
• Gene ID (NCBI): 664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.