BNIP3L Polyklonaler Antikörper

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• KD/KO Validated

BNIP3L Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 12986-1-AP

Synonyme

Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, BNIP3A, BNIP3H, NIP3L

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  WB analysis using 12986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12986-1-AP (BNIP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12986-1-AP (BNIP3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 12986-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12986-1-AP (BNIP3L antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12986-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12986-1-AP (BNIP3L antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, Mausnierengewebe, Raji-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 4 publications below

Produktinformation

12986-1-AP bindet in WB, IHC, ELISA BNIP3L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BNIP3L fusion protein Ag3624
• Vollständiger Name: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC001559
• Gene symbol: BNIP3L
• Gene ID (NCBI): 665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BNIP3L, also named as BNIP3A, BNIP3H, NIX and NIP3L. Bnip3L is one of the BH3-containing pro-apoptotic proteins and that it targets the mitochondria when inducing apoptosis. It may function as a tumor suppressor. BNIP3L has Dimer form with MW ~70 kDa.

Publikationen

Species	Application	Title
human	WB	Autophagy; Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.; Authors - Hongyu Li
mouse	WB	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai ; KO Validated
human	WB	Cell Rep; Proline restores mitochondrial function and reverses aging hallmarks in senescent cells; Authors - Debanik Choudhury
mouse	WB	Cell Rep; Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.; Authors - Tingting Fu
human	IHC	Neuromuscul Disord; Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies.; Authors - Shiro Matsubara
rat	WB	Int J Mol Sci; In Vivo Metabolic Responses to Different Formulations of Amino Acid Mixtures for the Treatment of Phenylketonuria (PKU).; Authors - Nadia Giarratana