BNIP3L Monoklonaler Antikörper

BNIP3L Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

1F6A3

Kat-Nr. : 68118-1-Ig

Synonyme

BNIP3H, BNIP3A, BNIP, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, Adenovirus E1B19K-binding protein B5

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  WB analysis using 68118-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of human prostate cancer using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68118-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MOLT-4-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Rattennierengewebe, humanes Prostatakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

68118-1-Ig bindet in WB, IHC, IF/ICC, ELISA BNIP3L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BNIP3L fusion protein Ag31547
• Vollständiger Name: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 35 kD
• GenBank-Zugangsnummer: BC001559
• Gene symbol: BNIP3L
• Gene ID (NCBI): 665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BNIP3L/Nix is a mitochondrial protein from the outer membrane that belongs to the BH3-only protein from the BCL2 family. BNIP3L was initially recognized as a proapoptotic protein with milder efficacy in inducing apoptosis compared to other proteins in this family. These phenotypes raised concerns about the molecular function of BNIP3L besides inducing apoptosis. Moreover, studies have shown that BNIP3L is a 24-kDa protein, which is predominantly expressed as a 48-kDa dimer. When analyzed by SDS-PAGE, BNIP3 migrates predominantly as a about 60 kDa dimer in addition to the 30 kDa monomer. (PMID: 34930907, PMID: 32286918)

Publikationen

Species	Application	Title
mouse	IF	J Zhejiang Univ Sci B; Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification; Authors - Tiantian Ge
mouse	WB	Eur J Neurosci; S-9-PAHSA Attenuates Aβ Accumulation and Improves Cognitive Deficits by Promoting Mitochondrial Autophagy in 5xFAD Mice; Authors - Chenyu Lu
human	WB	Autophagy; Bunyavirus SFTSV nucleoprotein exploits tufm-mediated mitophagy to impair antiviral innate immunity; Authors - Wen-Kang Zhang
monkey	WB	PLoS Pathog; Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation; Authors - Zhe Sun
human	WB	J Transl Med; Procyanidin C1 ameliorates acidic pH stress induced nucleus pulposus degeneration through SIRT3/FOXO3-mediated mitochondrial dynamics; Authors - Wenbin Hua