BOLA1 Polyklonaler Antikörper

BOLA1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 18017-1-AP

Synonyme

bolA homolog 1 (E. coli), BolA like protein 1, BOLA1, CGI 143, hBolA, RP11 196G18.18

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  WB analysis using 18017-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18017-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18017-1-AP

  IP result of anti-BOLA1 (IP:18017-1-AP, 4ug; Detection:18017-1-AP 1:800) with HepG2 cells lysate 3480ug.

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  IHC staining of human lung using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18017-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18017-1-AP

  Immunofluorescent analysis of HepG2 cells using 18017-1-AP (BOLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

18017-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BOLA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BOLA1 fusion protein Ag12565
• Vollständiger Name: bolA homolog 1 (E. coli)
• Berechnetes Molekulargewicht: 137 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC063405
• Gene symbol: BOLA1
• Gene ID (NCBI): 51027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Biomed Res Int; Prognostic Values of BolA Family Member Expression in Hepatocellular Carcinoma; Authors - Dong Wang
mouse	WB	bioRxiv; Proteome profiling identifies a link between the mitochondrial pathways and host-microbial sensor ELMO1 following Salmonella infection; Authors - Sajan C Achi