• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BORIS Monoklonaler Antikörper

BORIS Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B12E2

Kat-Nr. : 60079-1-PBS

Synonyme

CTCFL, 2B12E2, CCCTC-binding factor, CT27, CTCF paralog

Filter:
  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of SMMC-7721 using 60079-1-Ig (same clone as 60079-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

60079-1-PBS bindet in WB, IF/ICC, Indirect ELISA BORIS und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen BORIS fusion protein Ag1003
Vollständiger Name CCCTC-binding factor (zinc finger protein)-like
Berechnetes Molekulargewicht 82 kDa, 75 kDa
Beobachtetes Molekulargewicht 75 kDa
GenBank-ZugangsnummerBC130486
Gene symbol BORIS/CTCFL
Gene ID (NCBI) 140690
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.