BOULE Polyklonaler Antikörper

BOULE Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13720-1-PBS

Synonyme

BOLL, Protein boule-like

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  WB analysis using 13720-1-AP (same clone as 13720-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13720-1-AP (BOULE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  IP result of anti-BOULE (IP:13720-1-AP, 4ug; Detection:13720-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 13720-1-AP (BOULE antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13720-1-PBS bindet in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA BOULE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BOULE fusion protein Ag4662
• Vollständiger Name: bol, boule-like (Drosophila)
• Berechnetes Molekulargewicht: 283 aa, 31 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC033674
• Gene symbol: BOULE
• Gene ID (NCBI): 66037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).