BPIL1 Polyklonaler Antikörper

BPIL1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13461-2-AP

Synonyme

Bactericidal/permeability-increasing protein-like 1, BPI fold-containing family B member 2, BPIFB2, BPI-like 1, C20orf184

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  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13461-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13461-2-AP (BPIL1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.

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  IF Staining of A549 using 13461-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using BPIL1 antibody (13461-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:800-1:3200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

13461-2-AP bindet in WB, IHC, IF/ICC, ELISA BPIL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BPIL1 fusion protein Ag4051
• Vollständiger Name: bactericidal/permeability-increasing protein-like 1
• Berechnetes Molekulargewicht: 458 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC034415
• Gene symbol: BPIL1
• Gene ID (NCBI): 80341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bactericidal/permeability-increasing protein-like 1 (BPIL1, also known as LPLUNC2 or BPIFB2) is a member of the lipid transfer (LT)/lipopolysaccharide binding protein (LBP) family. LT/LBP proteins are structurally related proteins capable of binding phospholipids and lipopolysaccharides (PMID: 12185532). The gene of BPIL1 maps to Chromosome 20q11, and encodes a 458-amino acid protein with a calculated molecular weight of 49 kDa. It is highly expressed in hypertrophic tonsils. BPIL1 has been identified as a nasal mucous protein that may be involved in immune response in the nose against microbial infections (PMID: 15996010). Reduced expression of antimicrobial PLUNC proteins (LPLUNC1 and LPLUNC2) has been reported in nasal polyp tissues of patients with chronic rhinosinusitis (PMID: 22676062).

Publikationen

Species	Application	Title
human	WB,IHC	Allergy; Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis.; Authors - Seshadri S S
human	WB	J Proteome Res; Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.; Authors - Foster Matthew W MW
human	IHC	J Immunol Res; Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.; Authors - Yanran Huang