BPTF Polyklonaler Antikörper

BPTF Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 28016-1-AP

Synonyme

BPTF, FAC1, FALZ, Fetal Alz 50 clone 1 protein, Fetal Alzheimer antigen, NURF301

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  IHC staining of human breast cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28016-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28016-1-AP (BPTF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28016-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BPTF antibody (28016-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

28016-1-AP bindet in WB, IHC, IF/ICC, ChIP, ELISA BPTF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BPTF fusion protein Ag27761
• Vollständiger Name: bromodomain PHD finger transcription factor
• Berechnetes Molekulargewicht: 338 kDa
• GenBank-Zugangsnummer: NM_004459
• Gene symbol: BPTF
• Gene ID (NCBI): 2186
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bromodomain PHD finger transcription factor (BPTF), a histone-binding component of NURF (nucleosome-remodeling factor), plays an important role in catalyzing ATP-dependent nucleosome sliding and facilitating transcription of chromatin. It has been reported that BPTF maybe a novel and potential therapeutic target in hepatocellular carcinoma. BPTF may also regulate transcription through direct binding to DNA or transcription factors.(PMID: 30419422)

Publikationen

Species	Application	Title
human	WB,IHC,ChIP	Biomolecules; Lumbrokinase Extracted from Earthworms Synergizes with Bevacizumab and Chemotherapeutics in Treating Non-Small Cell Lung Cancer by Targeted Inactivation of BPTF/VEGF and NF-κB/COX-2 Signaling; Authors - Chunyu Hua