BRCA1 Monoklonaler Antikörper

BRCA1 Monoklonal Antikörper für ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

20D7G6

Kat-Nr. : 66267-1-Ig

Synonyme

BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53

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Galerie der Validierungsdaten

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (BRCA1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (BRCA1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 66267-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66267-1-Ig (BRCA1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

Empfohlene Verdünnung

Anwendung	Verdünnung
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

66267-1-Ig bindet in IF,ELISA BRCA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BRCA1 fusion protein Ag19178
• Vollständiger Name: breast cancer 1, early onset
• Berechnetes Molekulargewicht: 1863 aa, 208 kDa
• Beobachtetes Molekulargewicht: 207 kDa
• GenBank-Zugangsnummer: BC115037
• Gene symbol: BRCA1
• Gene ID (NCBI): 672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.

Publikationen

Species	Application	Title
human	IF	Oncogene; RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.; Authors - Yang Yu