BRCA1 Rekombinanter Antikörper

BRCA1 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240285H6

Kat-Nr. : 83390-2-PBS

Synonyme

BRCC1, BROVCA1, EC:2.3.2.27, IRIS, PSCP

---

• 

  Sandwich ELISA standard curve of MP00425-2

  Sandwich ELISA standard curve of MP00425-2, Human BRCA1 Recombinant Matched Antibody Pair - PBS only. 83390-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17946. 83390-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

• 

  Sample test of MP00425-2

  The mean BRCA1 concentration was determined to be 6.24 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 5.16 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 5.29 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP00425-2

  Cytometric bead array standard curve of MP00425-2, BRCA1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83390-4-PBS. Detection antibody: 83390-2-PBS. Standard: Ag17946. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83390-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83390-2-PBS against Human BRCA1 was performed. The affinity constant is 0.325 nM.

---

Geprüfte Anwendungen

Produktinformation

83390-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test BRCA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BRCA1 fusion protein Ag17946
• Vollständiger Name: breast cancer 1, early onset
• Berechnetes Molekulargewicht: 1863 aa, 208 kDa
• GenBank-Zugangsnummer: BC115037
• Gene symbol: BRCA1
• Gene ID (NCBI): 672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.