BRCC3 Rekombinanter Antikörper

BRCC3 Rekombinant Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242931B9

Kat-Nr. : 85430-4-PBS

Synonyme

BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1-A complex subunit BRCC36, BRCC36, BRISC complex subunit BRCC36

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  WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  IP experiment of SK-BR-3 using 85430-4-RR (same clone as 85430-4-PBS)

  IP result of anti-BRCC3 (IP:85430-4-RR, 4ug; Detection:85430-4-RR 1:2000) with SK-BR-3 cells lysate 1280 ug. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 85430-4-RR (same clone as 85430-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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  IHC staining of Human placenta using 85430-4-RR (same clone as 85430-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human placenta tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85430-4-PBS bindet in WB, IHC, IP, Indirect ELISA BRCC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BRCC3 fusion protein Ag7584
• Vollständiger Name: BRCA1/BRCA2-containing complex, subunit 3
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC002999
• Gene symbol: BRCC3
• Gene ID (NCBI): 79184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). BRCC3 is associated with G2/M arrest in breast cancer cells and DNA damage. The expression of BRCC3 is associated with increased cell proliferation. (PMID: 23246432, PMID: 30272359)