BRD2 Rekombinanter Antikörper

BRD2 Rekombinant Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250453G5

Kat-Nr. : 85990-2-PBS

Synonyme

bromodomain containing 2, Bromodomain-containing protein 2, D6S113E, FSH, FSRG1

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  WB analysis using 85990-2-RR (same clone as 85990-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85990-2-RR (BRD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85990-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 85990-2-RR (same clone as 85990-2-PBS)

  IP result of anti-BRD2 (IP:85990-2-RR, 4ug; Detection:85990-2-RR 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 85990-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 85990-2-RR (same clone as 85990-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85990-2-RR (BRD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85990-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85990-2-RR (same clone as 85990-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BRD2 antibody (85990-2-RR, Clone: 250453G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85990-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85990-2-PBS bindet in WB, IF/ICC, IP, Indirect ELISA BRD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BRD2 fusion protein Ag17589
• Vollständiger Name: bromodomain containing 2
• Berechnetes Molekulargewicht: 836 aa, 92 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC063840
• Gene symbol: BRD2
• Gene ID (NCBI): 6046
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD2 (Bromodomain-containing protein 2), also known as RING3, is a member of the Bromodomain and Extra-Terminal (BET) family of proteins. These proteins contain two bromodomains and one extra-terminal domain. BRD2 primarily localizes to the nucleus and functions as an atypical protein kinase. Its main role involves the activation of E2F1 and E2F2 proteins, facilitating the production of proteins essential for the G1/S phase transition of the cell cycle. BRD2 specifically recognizes acetylated histones through its bromodomains, particularly acetylated lysine residues on histones H3 and H4. This interaction allows BRD2 to recruit various transcription factors, co-activators, and repressors, thereby modulating transcriptional activity. BRD2 plays a crucial role in chromatin remodeling by recruiting proteins such as histone deacetylases. It is highly expressed during neural tube development and closure, making it essential for neurogenesis and embryogenesis.(PMID: 33462181, PMID: 37926722)