BRD3 Polyklonaler Antikörper

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• KD/KO Validated

BRD3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11859-1-PBS

Synonyme

Bromodomain-containing protein 3, KIAA0043, ORFX, RING3 like protein, RING3L

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  WB analysis using 11859-1-AP (same clone as 11859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 11859-1-AP (same clone as 11859-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

  IP result of anti-BRD3 (IP:11859-1-AP, 4ug; Detection:11859-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11859-1-AP (same clone as 11859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11859-1-AP (same clone as 11859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11859-1-AP (BRD3 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11859-1-AP (same clone as 11859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11859-1-AP (BRD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11859-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA BRD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRD3 fusion protein Ag2433
• Vollständiger Name: bromodomain containing 3
• Berechnetes Molekulargewicht: 556 aa, 61 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa
• GenBank-Zugangsnummer: BC032124
• Gene symbol: BRD3
• Gene ID (NCBI): 8019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).