BRD4 Polyklonaler Antikörper

BRD4 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 28486-1-AP

Synonyme

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

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Galerie der Validierungsdaten

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  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 28486-1-AP

  IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, Jurkat-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, Ramos-Zellen, T-47D-Zellen
• Erfolgreiche IP: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

28486-1-AP bindet in WB, IP, CoIP, ELISA BRD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: bromodomain containing 4
• Berechnetes Molekulargewicht: 1362 aa, 152 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC035266
• Gene symbol: BRD4
• Gene ID (NCBI): 23476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members.

Publikationen

Species	Application	Title
human	WB	bioRxiv; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination; Authors - Yi-Chang Wang
human,mouse	WB,CoIP	Biochem Pharmacol; BRD4 promotes hepatic stellate cells activation and hepatic fibrosis via mediating P300/H3K27ac/PLK1 axis; Authors - Miao Cheng ; KD Validated
human	WB	Cell Rep; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination; Authors - Yi-Chang Wang