BRD4 Polyklonaler Antikörper

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• KD/KO Validated

BRD4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28486-1-PBS

Synonyme

Protein HUNK1, MCAP, HUNKI, Bromodomain-containing protein 4, bromodomain containing 4

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  WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 28486-1-AP (same clone as 28486-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28486-1-AP (same clone as 28486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 28486-1-AP (same clone as 28486-1-PBS)

  IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28486-1-AP (same clone as 28486-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody (28486-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28486-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA BRD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: bromodomain containing 4
• Berechnetes Molekulargewicht: 1362 aa, 152 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC035266
• Gene symbol: BRD4
• Gene ID (NCBI): 23476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. The molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).