BRD4 Monoklonaler Antikörper

BRD4 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

2F7E2

Kat-Nr. : 67374-2-Ig

Synonyme

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

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  WB analysis using 67374-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67374-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• ChIP: See 2 publications below

Produktinformation

67374-2-Ig bindet in WB, ChIP, ELISA BRD4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BRD4 fusion protein Ag27545
• Vollständiger Name: bromodomain containing 4
• Berechnetes Molekulargewicht: 1362 aa, 152 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC035266
• Gene symbol: BRD4
• Gene ID (NCBI): 23476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Bioengineered; Pathological stage-associated non-coding RNA long intergenic non-protein coding RNA 1234 (LINC01234) participation in cell cycle regulation in adrenocortical carcinoma through bromodomain-containing protein 4 (BRD4) expression mediation via sponging microRNA (miR)-140-3p.; Authors - Dan-Dan Zhu
human	WB,ChIP	Curr Med Sci; ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer; Authors - Yang-Kai Jiang
human		J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan
mouse	WB	Nat Commun; MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition; Authors - Fan Tong
human,rat	WB	Tissue Cell; Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization; Authors - Lei Zhu ; KD Validated
human	ChIP	Cell; De novo assembly of nuclear stress bodies rearranges and enhances NFIL3 to restrain acute inflammatory responses; Authors - Xiao-Qi Liu