BRD7 Rekombinanter Antikörper

BRD7 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250776B2

Kat-Nr. : 86176-2-RR

Synonyme

BP75, Bromodomain-containing protein 7, CELTIX1, NAG4, Protein CELTIX 1

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  WB analysis using 86176-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 86176-2-RR

  PC-3 cells were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 86176-2-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, COLO 320-Zellen, Mauslungengewebe, PC-3-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86176-2-RR bindet in WB, ELISA BRD7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BRD7 fusion protein Ag1461
• Vollständiger Name: bromodomain containing 7
• Berechnetes Molekulargewicht: 651 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74-78 kDa
• GenBank-Zugangsnummer: BC050728
• Gene symbol: BRD7
• Gene ID (NCBI): 29117
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.