BRD8 Monoklonaler Antikörper

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BRD8 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5B6D6

Kat-Nr. : 60121-1-PBS

Synonyme

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

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  WB analysis of HeLa using 60121-1-Ig (same clone as 60121-1-PBS)

  WB result of BRD8 antibody (60121-1-Ig, 1:8000) with si-Control and si-BRD8 transfected HeLa cells.. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60121-1-Ig (same clone as 60121-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60121-1-Ig (BRD8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HEK-293 cells using 60121-1-Ig(BRD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60121-1-Ig (same clone as 60121-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD8 antibody (60121-1-Ig, Clone: 5B6D6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60121-1-PBS bindet in WB, IHC, IF/ICC, ELISA BRD8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BRD8 fusion protein Ag0790
• Vollständiger Name: bromodomain containing 8
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC008039
• Gene symbol: BRD8
• Gene ID (NCBI): 10902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].