BRE Polyklonaler Antikörper

BRE Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11702-1-PBS

Synonyme

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  WB analysis using 11702-1-AP (same clone as 11702-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11702-1-AP (BRE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 11702-1-AP (same clone as 11702-1-PBS)

  IP result of anti-BRE (IP:11702-1-AP, 4ug; Detection:11702-1-AP 1:1000) with HeLa cells lysate 2320 ug. This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11702-1-AP (same clone as 11702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11702-1-AP (same clone as 11702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11702-1-AP (BRE antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11702-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11702-1-PBS bindet in WB, IHC, IP, Indirect ELISA BRE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRE fusion protein Ag2256
• Vollständiger Name: brain and reproductive organ-expressed (TNFRSF1A modulator)
• Berechnetes Molekulargewicht: 383 aa, 44 kDa
• Beobachtetes Molekulargewicht: 40-44 kDa
• GenBank-Zugangsnummer: BC001251
• Gene symbol: BRE
• Gene ID (NCBI): 9577
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.