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BRF2 Polyklonaler Antikörper

BRF2 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12056-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HT-1080 cells using BRF2 Polyclonal antibody (12056-1-AP)

    WB analysis of HT-1080 using 12056-1-AP (same clone as 12056-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using BRF2 Polyclonal antibody (12056-1-AP)

    WB analysis of DU 145 using 12056-1-AP (same clone as 12056-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12056-1-PBS bindet in WB, Indirect ELISA BRF2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BRF2 fusion protein Ag2686
Vollständiger Name BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
Berechnetes Molekulargewicht 47 kDa
Beobachtetes Molekulargewicht 50-60 kDa
GenBank-ZugangsnummerBC010648
Gene symbol BRF2
Gene ID (NCBI) 55290
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.