MPC2 Polyklonaler Antikörper

MPC2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 20049-1-AP

Synonyme

Brain protein 44, BRP44, Mitochondrial pyruvate carrier 2

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  WB analysis of mouse brain using 20049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20049-1-AP (MPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 20049-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 20049-1-AP (MPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 20049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20049-1-AP (MPC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20049-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20049-1-AP (MPC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, LNCaP-Zellen, Maushirngewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

20049-1-AP bindet in WB, IHC, IF/ICC, ELISA MPC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: brain protein 44
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: NM_015415
• Gene symbol: MPC2
• Gene ID (NCBI): 25874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRP44 belongs to the UPF0041 (0-44) family. The antibody is specific to BRP44.

Publikationen

Species	Application	Title
human	WB	Curr Biol; Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.; Authors - Ridhima Gomkale
human	WB	Mol Cancer; 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants.; Authors - Eumorphia G Konstantakou
mouse	WB	J Biol Chem; Mitochondrial pyruvate carrier blockade results in decreased osteoclastogenesis and bone resorption via regulating mitochondrial energy production.; Authors - Qian Guo
human	WB	Hum Mol Genet; Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants; Authors - Stephanie A Zlatic
human	IF	Life Sci; Mitochondrial pyruvate carrier 2 mediates mitochondrial dysfunction and apoptosis in high glucose-treated podocytes.; Authors - Jun Feng
rat	WB,IF	Brain Res Bull; Downregulation of mitochondrial pyruvate carrier 2 aggravates neuronal injury in the cortex following cerebral ischemia in rat.; Authors - Yue Wang