BRPF1 Polyklonaler Antikörper

BRPF1 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30186-1-PBS

Synonyme

Protein Br140, Peregrin, Bromodomain and PHD finger-containing protein 1, BR140

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  WB analysis using 30186-1-AP (same clone as 30186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30186-1-AP (BRPF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRPF1 antibody (30186-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 30186-1-AP (same clone as 30186-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human BRPF1 (30186-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 30186-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30186-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA BRPF1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRPF1 fusion protein Ag32828
• Vollständiger Name: bromodomain and PHD finger containing, 1
• Berechnetes Molekulargewicht: 1220 aa, 138 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC053851
• Gene symbol: BRPF1
• Gene ID (NCBI): 7862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).