BRSK1 Polyklonaler Antikörper

BRSK1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12673-1-PBS

Synonyme

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  WB analysis using 12673-1-AP (same clone as 12673-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12673-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12673-1-AP (same clone as 12673-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12673-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12673-1-AP (same clone as 12673-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12673-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12673-1-AP (same clone as 12673-1-PBS)

  IP result of anti-BRSK1 (IP:12673-1-AP, 4ug; Detection:12673-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 12673-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12673-1-AP (same clone as 12673-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12673-1-AP (BRSK1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12673-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12673-1-PBS bindet in WB, IHC, IP, Indirect ELISA BRSK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRSK1 fusion protein Ag3357
• Vollständiger Name: BR serine/threonine kinase 1
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC016681
• Gene symbol: BRSK1
• Gene ID (NCBI): 84446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRSK1(Brain-specific serine/threonine-protein kinase 1) is also named as KIAA1811, SAD1, SADB and belongs to the SNF1 subfamily. BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. BRSK1 is a serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop. (PMID:22020259). It has 3 isoforms produced by alternative splicing.