BRSK1 Rekombinanter Antikörper

BRSK1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251341A11

Kat-Nr. : 86462-1-RR

Synonyme

BR serine/threonine kinase 1, BR serine/threonine-protein kinase 1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, EC:2.7.11.1

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  WB analysis using 86462-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86462-1-RR (BRSK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 86462-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 86462-1-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 86462-1-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 86462-1-RR (BRSK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86462-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86462-1-RR against Human BRSK1 were performed. The affinity constant is 0.173 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Ratten-Magengewebe, Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86462-1-RR bindet in WB, IHC, ELISA BRSK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BRSK1 fusion protein Ag3357
• Vollständiger Name: BR serine/threonine kinase 1
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC016681
• Gene symbol: BRSK1
• Gene ID (NCBI): 84446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRSK1(Brain-specific serine/threonine-protein kinase 1) is also named as KIAA1811, SAD1, SADB and belongs to the SNF1 subfamily. BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. BRSK1 is a serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop. (PMID:22020259). It has 3 isoforms produced by alternative splicing.