BRWD1 Polyklonaler Antikörper

BRWD1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29293-1-PBS

Synonyme

Bromodomain and WD repeat-containing protein 1, C21orf107, N143, WD repeat containing protein 9, WD repeat-containing protein 9

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  WB analysis using 29293-1-AP (same clone as 29293-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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  WB analysis using 29293-1-AP (same clone as 29293-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29293-1-AP (same clone as 29293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29293-1-AP (same clone as 29293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29293-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29293-1-PBS bindet in WB, IHC, Indirect ELISA BRWD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRWD1 fusion protein Ag30902
• Vollständiger Name: bromodomain and WD repeat domain containing 1
• Berechnetes Molekulargewicht: 2320 aa, 263 kDa
• Beobachtetes Molekulargewicht: 263 kDa
• GenBank-Zugangsnummer: BC064602
• Gene symbol: BRWD1
• Gene ID (NCBI): 54014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRWD1 (Bromodomain and WD repeat domain containing 1, also known as WDR9) has a predicted molecular weight of 263 kDa and contains tandem BROMO domains and WD40 repeats (PMID:12889071). One of the remarkable functions of BRWD1 was the coordinated repression of MYC and MYC target genes. At the Myc/MYC locus, in both mice and humans, BRWD1 specififically repressed distal enhancers, which are associated with lineage specifific expression (PMID:30250168). In addition,BRWD1 as a key epigenomic mediator of normal neurodevelopment and an important contributor to DS (Down syndrome)-related phenotypes (PMID:36289231).