BSAP,PAX5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BSAP,PAX5 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 26709-1-AP

Synonyme

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5

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Galerie der Validierungsdaten

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  WB analysis using 26709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 26709-1-AP

  WB result of BSAP,PAX5 antibody (26709-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BSAP,PAX5 transfected Raji cells.

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  WB analysis of Raji using 26709-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 26709-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Ramos using 26709-1-AP

  IP result of anti-BSAP,PAX5 (IP:26709-1-AP, 4ug; Detection:26709-1-AP 1:1000) with Ramos cells lysate 3600 ug.

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  IHC staining of human lymphoma using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26709-1-AP (BSAP,PAX5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26709-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26709-1-AP (BSAP,PAX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Ramos using 26709-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.20 ug/test Anti-Human BSAP,PAX5 (26709-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche IP: Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 3 publications below

Produktinformation

26709-1-AP bindet in WB, IP, IHC, FC, ELISA BSAP,PAX5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BSAP,PAX5 fusion protein Ag25147
• Vollständiger Name: paired box 5
• Berechnetes Molekulargewicht: 391 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC156927
• Gene symbol: PAX5
• Gene ID (NCBI): 5079
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

Publikationen

Species	Application	Title
human	IHC	Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
human	IHC	J Cutan Pathol; Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases.; Authors - Jennifer S Ko
mouse	IHC	Mol Oncol; miR-126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis-associated colorectal cancer; Authors - Shuai Wu