BTD Polyklonaler Antikörper

BTD Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16330-1-PBS

Synonyme

EC:3.5.1.12, biotinidase, Biotinase

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  WB analysis using 16330-1-AP (same clone as 16330-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16330-1-AP (BTD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 16330-1-AP (same clone as 16330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16330-1-AP (same clone as 16330-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTD antibody (16330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16330-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA BTD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BTD fusion protein Ag9449
• Vollständiger Name: biotinidase
• Berechnetes Molekulargewicht: 543 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC012099
• Gene symbol: BTD
• Gene ID (NCBI): 686
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.