BTF3 Polyklonaler Antikörper

BTF3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15415-1-AP

Synonyme

OK/SW-cl.8, Nascent polypeptide-associated complex subunit beta, NAC-beta, NACB, BTF3b

---

• 

  WB analysis using 15415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15415-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15415-1-AP (BTF3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15415-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTF3 antibody (15415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HT-29-Zellen, HuH-7-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15415-1-AP bindet in WB, IHC, IF/ICC, ELISA BTF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BTF3 fusion protein Ag7701
• Vollständiger Name: basic transcription factor 3
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 18 kDa, 23 kDa
• GenBank-Zugangsnummer: BC008062
• Gene symbol: BTF3
• Gene ID (NCBI): 689
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Basic transcription factor 3 (BTF3), also known as nascent-polypeptide-associated complex (NAC) beta (NACB), belongs to the NAC-beta family. BTF3 serves as an oncogene and convey worse prognosis in gastric cancer, pancreatic cancer, osteosarcoma, cervical cancer, hypopharyngeal squamous cell carcinoma, prostate cancer , breast cancer. Due to alternative splicing, BTF3 is present in two different isoforms, BTF3a and BTF3b. BTF3a is the transcriptionally active form of BTF3, while the isoform lacking the first 44 amino acids of the BTF3a N-terminus, BTF3b, is transcriptionally inactive, two isoforms of BTF3, BTF3a and BTF3b corresponding to 23kDa and 18kDa(PMID: 23029305).