BTF3 Polyklonaler Antikörper

BTF3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15415-1-PBS

Synonyme

OK/SW-cl.8, Nascent polypeptide-associated complex subunit beta, NAC-beta, NACB, BTF3b

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  WB analysis using 15415-1-AP (same clone as 15415-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15415-1-PBS in a different storage buffer formulation.

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  WB analysis using 15415-1-AP (same clone as 15415-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15415-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15415-1-AP (same clone as 15415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15415-1-AP (BTF3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15415-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15415-1-AP (same clone as 15415-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTF3 antibody (15415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15415-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15415-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA BTF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BTF3 fusion protein Ag7701
• Vollständiger Name: basic transcription factor 3
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 18 kDa, 23 kDa
• GenBank-Zugangsnummer: BC008062
• Gene symbol: BTF3
• Gene ID (NCBI): 689
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Basic transcription factor 3 (BTF3), also known as nascent-polypeptide-associated complex (NAC) beta (NACB), belongs to the NAC-beta family. BTF3 serves as an oncogene and convey worse prognosis in gastric cancer, pancreatic cancer, osteosarcoma, cervical cancer, hypopharyngeal squamous cell carcinoma, prostate cancer , breast cancer. Due to alternative splicing, BTF3 is present in two different isoforms, BTF3a and BTF3b. BTF3a is the transcriptionally active form of BTF3, while the isoform lacking the first 44 amino acids of the BTF3a N-terminus, BTF3b, is transcriptionally inactive, two isoforms of BTF3, BTF3a and BTF3b corresponding to 23kDa and 18kDa(PMID: 23029305).