BTG2 Polyklonaler Antikörper

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• KD/KO Validated

BTG2 Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 22339-1-AP

Synonyme

BTG family, member 2, PC3, Protein BTG2

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  IHC staining of mouse small intestine using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22339-1-AP (BTG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22339-1-AP (BTG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22339-1-AP (BTG2 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 22339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22339-1-AP (BTG2 Antibody) at dilution of 1:50.

• 

  IF Staining of Neuro-2a using 22339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using BTG2 antibody (22339-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 22339-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human BTG2 (22339-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Neuro-2a using 22339-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human BTG2 (22339-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen, Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

22339-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA BTG2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BTG2 fusion protein Ag17819
• Vollständiger Name: BTG family, member 2
• Berechnetes Molekulargewicht: 158 aa, 17 kDa
• GenBank-Zugangsnummer: BC105949
• Gene symbol: BTG2
• Gene ID (NCBI): 7832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein BTG2, the anti-proliferative human homolog of murine Pc3/Tis21, belongs to the BTG/Tob family. This family has structurally related proteins that appear to have anti-proliferative properties. It has been demonstrated that BTG2 is p53-inducible and is involved in cell cycle control and cellular response to DNA damage (PMID: 8944033). As a transcriptional co-regulator, BTG2 has been shown to interact with CAF1 and POP2, and works as a co-activator of ERa-mediated transcription via a CCR4-like complex (PMID: 18974182).

Publikationen

Species	Application	Title
human	WB	Front Oncol; Transcriptomic Analyses Reveal B-Cell Translocation Gene 2 as a Potential Therapeutic Target in Ovarian Cancer.; Authors - Jia Wang ; KD Validated
mouse	WB,IF	FASEB J; MicroRNA-409-3p/BTG2 signaling axis improves impaired angiogenesis and wound healing in obese mice; Authors - Furkan Bestepe
mouse	WB	ACS Chem Biol; Epitranscriptomic N6-Methyladenosine Modification Is Required for Direct Lineage Reprogramming into Neurons.; Authors - Hwan Choi
human	WB	Front Mol Biosci; Development and Validation of a 7-Gene Inflammatory Signature Forecasts Prognosis and Diverse Immune Landscape in Lung Adenocarcinoma.; Authors - Aitao Nai
human	WB	J Oncol; lncRNA SNHG5 Modulates Endometrial Cancer Progression via the miR-25-3p/BTG2 Axis.; Authors - Shi Li
human	IHC	Dig Dis Sci; BTG2 Is Down-Regulated and Inhibits Cancer Stem Cell-Like Features of Side Population Cells in Hepatocellular Carcinoma.; Authors - Chen-Song Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, incubated with AF488 at room temperature for 2 h.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Ovarian tissue