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BTG2 Polyklonaler Antikörper
BTG2 Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 22339-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IHC | Maus-Dünndarmgewebe, humanes Nierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | Neuro-2a-Zellen |
| Erfolgreiche Detektion in FC (Intra) | SH-SY5Y-Zellen, Neuro-2a-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
Produktinformation
22339-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA BTG2 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | BTG2 fusion protein Ag17819 |
| Vollständiger Name | BTG family, member 2 |
| Berechnetes Molekulargewicht | 158 aa, 17 kDa |
| GenBank-Zugangsnummer | BC105949 |
| Gene symbol | BTG2 |
| Gene ID (NCBI) | 7832 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein BTG2, the anti-proliferative human homolog of murine Pc3/Tis21, belongs to the BTG/Tob family. This family has structurally related proteins that appear to have anti-proliferative properties. It has been demonstrated that BTG2 is p53-inducible and is involved in cell cycle control and cellular response to DNA damage (PMID: 8944033). As a transcriptional co-regulator, BTG2 has been shown to interact with CAF1 and POP2, and works as a co-activator of ERa-mediated transcription via a CCR4-like complex (PMID: 18974182).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IHC protocol for BTG2 antibody 22339-1-AP | Protokoll herunterladenl |
| IF protocol for BTG2 antibody 22339-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Front Oncol Transcriptomic Analyses Reveal B-Cell Translocation Gene 2 as a Potential Therapeutic Target in Ovarian Cancer.
| ||
FASEB J MicroRNA-409-3p/BTG2 signaling axis improves impaired angiogenesis and wound healing in obese mice | ||
ACS Chem Biol Epitranscriptomic N6-Methyladenosine Modification Is Required for Direct Lineage Reprogramming into Neurons. | ||
Front Mol Biosci Development and Validation of a 7-Gene Inflammatory Signature Forecasts Prognosis and Diverse Immune Landscape in Lung Adenocarcinoma. | ||
J Oncol lncRNA SNHG5 Modulates Endometrial Cancer Progression via the miR-25-3p/BTG2 Axis. | ||
Dig Dis Sci BTG2 Is Down-Regulated and Inhibits Cancer Stem Cell-Like Features of Side Population Cells in Hepatocellular Carcinoma. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tianyi (Verified Customer) (01-12-2024) | Antigen retrieval in TE buffer, incubated with AF488 at room temperature for 2 h.
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