BTK Polyklonaler Antikörper

BTK Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21581-1-AP

Synonyme

EC:2.7.10.2, BPK, B-cell progenitor kinase, ATK, AGMX1

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  WB analysis using 21581-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21581-1-AP (BTK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21581-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21581-1-AP (BTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 21581-1-AP

  IP result of anti-BTK (IP:21581-1-AP, 4ug; Detection:21581-1-AP 1:3000) with mouse spleen tissue lysate 1920 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HT-29-Zellen, NIH/3T3-Zellen, Ramos-Zellen
• Erfolgreiche IP: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

21581-1-AP bindet in WB, IHC, IP, ELISA BTK und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BTK fusion protein Ag16133
• Vollständiger Name: Bruton agammaglobulinemia tyrosine kinase
• Berechnetes Molekulargewicht: 659 aa, 76 kDa
• Beobachtetes Molekulargewicht: 70-76 kDa
• GenBank-Zugangsnummer: BC109079
• Gene symbol: BTK
• Gene ID (NCBI): 695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction.; Authors - Weijia Sun
human	WB	Int J Mol Med; Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.; Authors - Linlin Sheng
mouse	WB	Redox Biol; CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway; Authors - Chengli Liu
human	WB,IHC	J Cancer; Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma; Authors - Yan Wang
human	WB	Cell Death Discov; Tuning the B-CLL microenvironment: evidence for BAG3 protein- mediated regulation of stromal fibroblasts activity; Authors - Anna Basile