BTLA/CD272 Rekombinanter Antikörper

BTLA/CD272 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242133E5

Kat-Nr. : 84754-5-PBS

Synonyme

242133E5, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, Btla, CD272

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  WB analysis using 84754-5-RR (same clone as 84754-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84754-5-RR (BTLA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84754-5-PBS in a different storage buffer formulation.

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  IF Staining of A20 using 84754-5-RR (same clone as 84754-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using BTLA antibody (84754-5-RR, Clone: 242133E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84754-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84754-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84754-5-RR against Mouse BTLA were performed. The affinity constant is 1.12 nM.

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Geprüfte Anwendungen

Produktinformation

84754-5-PBS bindet in WB, IF/ICC, Indirect ELISA BTLA/CD272 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BTLA/CD272 fusion protein Eg1843
• Vollständiger Name: B and T lymphocyte associated
• Berechnetes Molekulargewicht: 34kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: AAI08964.1
• Gene symbol: Btla
• Gene ID (NCBI): 208154
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BTLA, or B and T lymphocyte attenuator, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.