BTLA/CD272 Rekombinanter Antikörper

BTLA/CD272 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242133A1

Kat-Nr. : 98246-2-RR

Synonyme

CD272, Btla, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133A1

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  FC experiment of mouse splenocytes using 98246-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

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  FC experiment of mouse splenocytes using 98246-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98246-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98246-2-RR against Mouse BTLA/CD272 were performed. The affinity constant is 0.441 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98246-2-RR bindet in FC BTLA/CD272 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: B and T lymphocyte associated
• Berechnetes Molekulargewicht: 34kDa
• GenBank-Zugangsnummer: AAI08964.1
• Gene symbol: Btla
• Gene ID (NCBI): 208154
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

BTLA, also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.