BTLA/CD272 Rekombinanter Antikörper

BTLA/CD272 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

241318H5

Kat-Nr. : CL594-98096

Synonyme

BTLA, 241318H5, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator

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  FC experiment of human PBMCs using CL594-98096

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 5 ul CoraLite®594 Anti-Human BTLA/CD272 Rabbit RecAb (CL594-98096, Clone: 241318H5) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL594-98096

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human BTLA/CD272 Rabbit RecAb (CL594-98096, Clone: 241318H5) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98096 bindet in FC BTLA/CD272 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: B and T lymphocyte associated
• Berechnetes Molekulargewicht: 289 aa, 33 kDa
• GenBank-Zugangsnummer: BC107091
• Gene symbol: BTLA
• Gene ID (NCBI): 151888
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

BTLA ( B and T lymphocyte attenuator), also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.