BTN2A2 Polyklonaler Antikörper

BTN2A2 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16482-1-PBS

Synonyme

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  WB analysis of MCF-7 using 16482-1-AP (same clone as 16482-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 16482-1-AP (same clone as 16482-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 16482-1-AP (same clone as 16482-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16482-1-AP (same clone as 16482-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16482-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16482-1-PBS bindet in WB, IF/ICC, Indirect ELISA BTN2A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BTN2A2 fusion protein Ag9603
• Vollständiger Name: butyrophilin, subfamily 2, member A2
• Berechnetes Molekulargewicht: 523 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC014021
• Gene symbol: BTN2A2
• Gene ID (NCBI): 10385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).