BTN3A1 Polyklonaler Antikörper

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• KD/KO Validated

BTN3A1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 25221-1-AP

Synonyme

CD277, BTF5, BT3.1

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  WB analysis of U-937 using 25221-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 25221-1-AP (BTN3A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25221-1-AP (BTN3A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 25221-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BTN3A1 antibody (25221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

25221-1-AP bindet in WB, IHC, IF/ICC, ELISA BTN3A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BTN3A1 fusion protein Ag18414
• Vollständiger Name: butyrophilin, subfamily 3, member A1
• Berechnetes Molekulargewicht: 513 aa, 58 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC121800
• Gene symbol: BTN3A1
• Gene ID (NCBI): 11119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BTN3A1, also named as Butyrophilin subfamily 3 member A1 or BTF5, is a 513 amino acid protein ,which contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 localizes in the cell membrane and belongs to the immunoglobulin superfamily. BTN/MOG family. BTN3A1 is detected on T-cells, natural killer cells, dendritic cells and macrophages. It is ubiquitously highly expressed in heart, pancreas and lung and moderately expressed in placenta, liver and muscle. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. It also regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. It exists as four isoforms. We detected a 48 kda isoform by western blot.

Publikationen

Species	Application	Title
human	WB	Nat Commun; The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells.; Authors - Barbara Castella ; KD Validated
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human	WB	Biochem Biophys Res Commun; Expression level of BTN3A1 on the surface of CD14+ monocytes is a potential predictor of γδ T cell expansion efficiency.; Authors - Mako Tomogane
human	WB, IHC	FEBS Open Bio; Comprehensive analysis of BTN3A1 in cancers: mining of omics data and validation in patient samples and cellular models.; Authors - Fan Liang