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BTN3A1 Polyklonaler Antikörper

BTN3A1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25221-1-PBS

Synonyme

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  • Western Blot (WB) analysis of U-937 cells using BTN3A1 Polyclonal antibody (25221-1-AP)

    WB analysis of U-937 using 25221-1-AP (same clone as 25221-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 25221-1-AP (BTN3A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25221-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using BTN3A1 Polyclonal antibody (25221-1-AP)

    WB analysis of Jurkat using 25221-1-AP (same clone as 25221-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25221-1-AP (BTN3A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25221-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using BTN3A1 Polyclonal antibody (25221-1-AP)

    IF Staining of U-251 using 25221-1-AP (same clone as 25221-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BTN3A1 antibody (25221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25221-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25221-1-PBS bindet in WB, IF/ICC, Indirect ELISA BTN3A1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BTN3A1 fusion protein Ag18414
Vollständiger Name butyrophilin, subfamily 3, member A1
Berechnetes Molekulargewicht 513 aa, 58 kDa
Beobachtetes Molekulargewicht 48 kDa
GenBank-ZugangsnummerBC121800
Gene symbol BTN3A1
Gene ID (NCBI) 11119
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BTN3A1, also named as Butyrophilin subfamily 3 member A1 or BTF5, is a 513 amino acid protein ,which contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 localizes in the cell membrane and belongs to the immunoglobulin superfamily. BTN/MOG family. BTN3A1 is detected on T-cells, natural killer cells, dendritic cells and macrophages. It is ubiquitously highly expressed in heart, pancreas and lung and moderately expressed in placenta, liver and muscle. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. It also regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. It exists as four isoforms. We detected a 48 kda isoform by western blot.