BUB1 Polyklonaler Antikörper

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BUB1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13330-1-PBS

Synonyme

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  WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13330-1-AP (same clone as 13330-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 13330-1-AP (same clone as 13330-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

  IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 13330-1-AP (same clone as 13330-1-PBS)

  Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13330-1-PBS bindet in WB, IF/ICC, Indirect ELISA BUB1 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BUB1 fusion protein Ag4138
• Vollständiger Name: budding uninhibited by benzimidazoles 1 homolog (yeast)
• Berechnetes Molekulargewicht: 1085 aa, 122 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 120 kDa
• GenBank-Zugangsnummer: BC028201
• Gene symbol: BUB1
• Gene ID (NCBI): 699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).