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BUB1 Rekombinanter Antikörper

BUB1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251580F8

Kat-Nr. : 86843-1-PBS

Synonyme

BUB1A, BUB1L, EC:2.7.11.1, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1

Filter:
  • Western Blot (WB) analysis of various lysates using BUB1 Recombinant monoclonal antibody (86843-1-RR)

    WB analysis using 86843-1-RR (same clone as 86843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86843-1-RR (BUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86843-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86843-1-PBS bindet in WB, Indirect ELISA BUB1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen BUB1 fusion protein Ag4138
Vollständiger Name budding uninhibited by benzimidazoles 1 homolog (yeast)
Berechnetes Molekulargewicht 1085 aa, 122 kDa
Beobachtetes Molekulargewicht120 kDa
GenBank-ZugangsnummerBC028201
Gene symbol BUB1
Gene ID (NCBI) 699
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).