- Featured Product
- KD/KO Validated
BUB1B Polyklonaler Antikörper
BUB1B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11504-2-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushodengewebe, HeLa-Zellen, humanes Hodengewebe, K-562-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Zervixkarzinomgewebe,  humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | SKOV-3-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| Immunhistochemie (IHC) | IHC : 1:250-1:1000 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 12 publications below | 
| IHC | See 3 publications below | 
| IF | See 5 publications below | 
Produktinformation
11504-2-AP bindet in WB, IHC, IF/ICC, ELISA BUB1B und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus | 
| In Publikationen genannte Reaktivität | human, Maus | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | BUB1B fusion protein Ag2053 | 
| Vollständiger Name | budding uninhibited by benzimidazoles 1 homolog beta (yeast) | 
| Berechnetes Molekulargewicht | 1050 aa, 120 kDa | 
| Beobachtetes Molekulargewicht | 120-130 kDa | 
| GenBank-Zugangsnummer | BC018739 | 
| Gene symbol | BubR1 | 
| Gene ID (NCBI) | 701 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3, and SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID: 22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications, including acetylation(PMID: 22167194). Defects in BUB1B are the cause of the premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1). Research has shown that a BUB1B isoform with a measured MW of about 65 kDa (PMID: 34620840).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for BUB1B antibody 11504-2-AP | Protokoll herunterladen | 
| IHC protocol for BUB1B antibody 11504-2-AP | Protokoll herunterladenl | 
| IF protocol for BUB1B antibody 11504-2-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. | ||
| Cancer Lett Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells. | ||
| Bioorg Chem Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors. | ||
| Eur J Med Chem Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents. | ||
| Int J Biol Sci The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. | 















