BUB1B Polyklonaler Antikörper

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BUB1B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 11504-2-AP

Synonyme

BubR1, Bub1A, BUB1beta, EC:2.7.11.1, hBUBR1

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  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11504-2-AP

  WB result of BubR1 antibody (11504-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BubR1 transfected HeLa cells.

• 

  WB analysis of K-562 using 11504-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11504-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11504-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11504-2-AP (BubR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using BubR1 antibody (11504-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, humanes Hodengewebe, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Produktinformation

11504-2-AP bindet in WB, IHC, IF/ICC, ELISA BUB1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BUB1B fusion protein Ag2053
• Vollständiger Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Berechnetes Molekulargewicht: 1050 aa, 120 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC018739
• Gene symbol: BubR1
• Gene ID (NCBI): 701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3, and SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID: 22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications, including acetylation(PMID: 22167194). Defects in BUB1B are the cause of the premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1). Research has shown that a BUB1B isoform with a measured MW of about 65 kDa (PMID: 34620840).

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu
human	WB	Cancer Lett; Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells.; Authors - Chuangyu Wen
human	WB	Bioorg Chem; Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors.; Authors - Yuru Liang
human	WB	Eur J Med Chem; Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents.; Authors - Pengfei Zhou
human	WB	Int J Biol Sci; The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.; Authors - Wei Xu