BubR1 Polyklonaler Antikörper

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• KD/KO Validated

BubR1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11504-2-AP

Synonyme

Bub1A, BUB1B, BUB1beta, BubR1, hBUBR1, MAD3L, Protein SSK1, SSK1

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11504-2-AP

  WB result of BubR1 antibody (11504-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BubR1 transfected HeLa cells.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11504-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11504-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11504-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using BubR1 antibody (11504-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, humanes Hodengewebe, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

11504-2-AP bindet in WB, IHC, IF, ELISA BubR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BubR1 fusion protein Ag2053
• Vollständiger Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Berechnetes Molekulargewicht: 1050 aa, 120 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC018739
• Gene symbol: BUB1B
• Gene ID (NCBI): 701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu
human	WB	Cancer Lett; Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells.; Authors - Chuangyu Wen
human	WB	Bioorg Chem; Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors.; Authors - Yuru Liang
human	WB	Eur J Med Chem; Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents.; Authors - Pengfei Zhou
human	WB	Int J Biol Sci; The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.; Authors - Wei Xu