BUB1B Monoklonaler Antikörper

BUB1B Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B2D5

Kat-Nr. : 60423-1-Ig

Synonyme

BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A

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  WB analysis using 60423-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60423-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HaCaT-Zellen, HEK-293-Zellen, HeLa-Zellen, Ramos-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60423-1-Ig bindet in WB, ELISA BUB1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BUB1B fusion protein Ag34182
• Vollständiger Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Berechnetes Molekulargewicht: 1050 aa, 120 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC018739
• Gene symbol: BubR1
• Gene ID (NCBI): 701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).